multidisciplinary

Human glioma samplesFrozen glioma tissue specimens with the IDH1 or IDH2 mutation were collected with informed consent from the following tissue source sites: Saint Joseph’s Hospital, the Luxembourg Institute of Health-NORLUX…

Y-shaped biomolecules called antibodies are scientific workhorse tools that researchers use to bind to and track specific proteins.Credit: Nemes Laszlo/Science Photo LibraryCatalogue entries for more than 100 antibodies sold by the…

Study participant details from the ARTEMIS TrialThe clinical trial protocol was reviewed and approved by The University of Texas MD Anderson Cancer Center Institutional Review Board and all participants provided written informed consent…

Ashkin, A., Dziedzic, J. M., Bjorkholm, J. E. & Chu, S. Observation of a single-beam gradient force optical trap for dielectric particles. Opt. Lett. 11, 288–290 (1986).Article  ADS  CAS  PubMed …

Binding screenThese experiments were performed by a commercial vendor (Eurofins) under contract with the National Institutes of Health (NIH). Membrane homogenates from stable cell lines or animal tissues expressing each protein were…

Animals and cell linesAnimal workMouse (Mus musculus) strains C57BL/6J (strain no. 000664) and Cdx2:CreERT2;APCfl/fl;KrasWT (strain no. 035169)50 were obtained from The Jackson Laboratory. Mice were housed at room temperature and ambient…

Cloning, cell culture and protein expressionN-terminally fused mEGFP constructs of TRPM8 were obtained using Gibson assembly to sub-clone TRPM8 genes into a pFastBac1 vector. Mutagenesis was carried out by either PCR with mutagenic…

MiceUnless noted otherwise, young female (8 weeks old) and male (4 weeks old) C57BL/6 mice were obtained from The Jackson Laboratory and old mice (18 months old) were acquired from the National Institute on Aging. For co-housing, mice…

A ‘cryosleep pod’ in the 1979 science-fiction film Alien.Credit: 20TH CENTURY FOX via AJ Pics/AlamyA familiar trope in science fiction is the cryopreserved time traveller, their body deep-frozen in suspended animation, then thawed and…

Nguyen, E. et al. Sequence modeling and design from molecular to genome scale with Evo. Science 386, eado9336 (2024).Article  CAS  PubMed  PubMed Central  Google Scholar  Merchant, A.…